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ym1 antibody  (STEMCELL Technologies Inc)

 
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    Structured Review

    STEMCELL Technologies Inc ym1 antibody
    MLKL in myofibroblasts affects the activity of macrophages. ( A ) The secretion of IL-6, NO and TNF-α in MLKL +/+ M1ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( B ) The secretion of IL-10, arginase and <t>Ym1</t> in MLKL +/+ M2ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( C )The skin wound tissues of MLKL +/+ and MLKL −/− mice were subjected to immunofluorescence staining for arginase (red), Ym1 (green) and nuclear (DAPI, blue) on the 5, 7 and 10th days after wound injury. Merge represents the composite picture of target protein and nuclear. The immunofluorescence staining was imaged by fluorescence microscopy (Zeiss LSM 800 laser, ×200 magnification), and the mean value of fluorescence intensity was used to perform calculation (a. u., arbitrary unit). ( D ) The protein expression of arginase and Ym1 in wound tissues of MLKL +/+ and MLKL −/− mice were determined by western blot. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean ± SD of multiple independent experiments and analyzed by Student t test or one-way ANOVA followed by Tukey’s multiple comparisons test ( n = 4–6). * P < 0.05, ** P < 0.01, *** P < 0.001. Scale label = 20 μm.
    Ym1 Antibody, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ym1 antibody/product/STEMCELL Technologies Inc
    Average 90 stars, based on 1 article reviews
    ym1 antibody - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "MLKL is involved in the regulation of skin wound healing and interplay between macrophages and myofibroblasts in mice"

    Article Title: MLKL is involved in the regulation of skin wound healing and interplay between macrophages and myofibroblasts in mice

    Journal: Scientific Reports

    doi: 10.1038/s41598-025-97729-2

    MLKL in myofibroblasts affects the activity of macrophages. ( A ) The secretion of IL-6, NO and TNF-α in MLKL +/+ M1ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( B ) The secretion of IL-10, arginase and Ym1 in MLKL +/+ M2ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( C )The skin wound tissues of MLKL +/+ and MLKL −/− mice were subjected to immunofluorescence staining for arginase (red), Ym1 (green) and nuclear (DAPI, blue) on the 5, 7 and 10th days after wound injury. Merge represents the composite picture of target protein and nuclear. The immunofluorescence staining was imaged by fluorescence microscopy (Zeiss LSM 800 laser, ×200 magnification), and the mean value of fluorescence intensity was used to perform calculation (a. u., arbitrary unit). ( D ) The protein expression of arginase and Ym1 in wound tissues of MLKL +/+ and MLKL −/− mice were determined by western blot. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean ± SD of multiple independent experiments and analyzed by Student t test or one-way ANOVA followed by Tukey’s multiple comparisons test ( n = 4–6). * P < 0.05, ** P < 0.01, *** P < 0.001. Scale label = 20 μm.
    Figure Legend Snippet: MLKL in myofibroblasts affects the activity of macrophages. ( A ) The secretion of IL-6, NO and TNF-α in MLKL +/+ M1ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( B ) The secretion of IL-10, arginase and Ym1 in MLKL +/+ M2ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( C )The skin wound tissues of MLKL +/+ and MLKL −/− mice were subjected to immunofluorescence staining for arginase (red), Ym1 (green) and nuclear (DAPI, blue) on the 5, 7 and 10th days after wound injury. Merge represents the composite picture of target protein and nuclear. The immunofluorescence staining was imaged by fluorescence microscopy (Zeiss LSM 800 laser, ×200 magnification), and the mean value of fluorescence intensity was used to perform calculation (a. u., arbitrary unit). ( D ) The protein expression of arginase and Ym1 in wound tissues of MLKL +/+ and MLKL −/− mice were determined by western blot. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean ± SD of multiple independent experiments and analyzed by Student t test or one-way ANOVA followed by Tukey’s multiple comparisons test ( n = 4–6). * P < 0.05, ** P < 0.01, *** P < 0.001. Scale label = 20 μm.

    Techniques Used: Activity Assay, Immunofluorescence, Staining, Fluorescence, Microscopy, Expressing, Western Blot, Control, Software

    MLKL in myofibroblasts affects macrophages activities through PGE 2 . ( A ) The production of IL-6, NO and TNF-α in MLKL +/+ M1 macrophages after MLKL −/− MFbCM treatment or MLKL −/− MFbCM plus PGE 2 treatment. ( B ) The production of IL-10, arginase and Ym1 in MLKL +/+ M2ø after MLKL −/− MFbCM treatment or MLKL −/− MFbCM plus PGE 2 treatment. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean ± SD and were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test ( n = 4–6). * P < 0.05, ** P < 0.01, *** P < 0.001. ns not significant.
    Figure Legend Snippet: MLKL in myofibroblasts affects macrophages activities through PGE 2 . ( A ) The production of IL-6, NO and TNF-α in MLKL +/+ M1 macrophages after MLKL −/− MFbCM treatment or MLKL −/− MFbCM plus PGE 2 treatment. ( B ) The production of IL-10, arginase and Ym1 in MLKL +/+ M2ø after MLKL −/− MFbCM treatment or MLKL −/− MFbCM plus PGE 2 treatment. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean ± SD and were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test ( n = 4–6). * P < 0.05, ** P < 0.01, *** P < 0.001. ns not significant.

    Techniques Used: Control, Software

    Antibodies used in Immunofluorescence staining.
    Figure Legend Snippet: Antibodies used in Immunofluorescence staining.

    Techniques Used: Immunofluorescence, Staining, Concentration Assay

    Antibodies used in Western blot.
    Figure Legend Snippet: Antibodies used in Western blot.

    Techniques Used: Western Blot, Concentration Assay



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    MLKL in myofibroblasts affects the activity of macrophages. ( A ) The secretion of IL-6, NO and TNF-α in MLKL +/+ M1ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( B ) The secretion of IL-10, arginase and <t>Ym1</t> in MLKL +/+ M2ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( C )The skin wound tissues of MLKL +/+ and MLKL −/− mice were subjected to immunofluorescence staining for arginase (red), Ym1 (green) and nuclear (DAPI, blue) on the 5, 7 and 10th days after wound injury. Merge represents the composite picture of target protein and nuclear. The immunofluorescence staining was imaged by fluorescence microscopy (Zeiss LSM 800 laser, ×200 magnification), and the mean value of fluorescence intensity was used to perform calculation (a. u., arbitrary unit). ( D ) The protein expression of arginase and Ym1 in wound tissues of MLKL +/+ and MLKL −/− mice were determined by western blot. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean ± SD of multiple independent experiments and analyzed by Student t test or one-way ANOVA followed by Tukey’s multiple comparisons test ( n = 4–6). * P < 0.05, ** P < 0.01, *** P < 0.001. Scale label = 20 μm.
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    Image Search Results


    (A) Control and Het KI lung sections were immunostained for F4/80 (control, 10 week-old male; Het KI, 10 week-old male) and Ym1 (control, 9 week-old female; Het KI, 9 week-old male). Results are shown at 20x magnification. (B) Ultrastructural analysis of lungs from control (12 week-old male) and Het KI (12 week-old male) mice. (C) Immunostaining of lungs from control (10 week-old male) and Het KI (10 week-old male) mice with surfactant-associated proteins SP-A and SP-C. Results are shown at 20x magnification.

    Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    Article Title: Lethal eosinophilic crystalline pneumonia in mice expressing a stabilized Csf2 mRNA

    doi: 10.1096/fj.202300757R

    Figure Lengend Snippet: (A) Control and Het KI lung sections were immunostained for F4/80 (control, 10 week-old male; Het KI, 10 week-old male) and Ym1 (control, 9 week-old female; Het KI, 9 week-old male). Results are shown at 20x magnification. (B) Ultrastructural analysis of lungs from control (12 week-old male) and Het KI (12 week-old male) mice. (C) Immunostaining of lungs from control (10 week-old male) and Het KI (10 week-old male) mice with surfactant-associated proteins SP-A and SP-C. Results are shown at 20x magnification.

    Article Snippet: Sections were incubated either with goat anti-mouse Ym1 (Chitinase 3-like 3/ECF-L) antibody (AF2446, R&D Systems, Minneapolis, MN) at a dilution of 1:500, followed by biotinylated anti-goat IgG (H+L) secondary antibody (1:2000; Vector Laboratories), rat anti-mouse F4/80 monoclonal antibody (BM8; 123102, BioLegend San Diego, CA) at a dilution of 1:500 followed by biotinylated rabbit anti-rat IgG secondary antibody (1:500; Vector Laboratories), or goat polyclonal SP-A antibody (sc-7699, Santa Cruz Biotechnology, Dallas, TX) at a dilution of 1:100 followed by biotinylated horse anti-goat IgG secondary antibody (1:500; Vector Laboratories).

    Techniques: Control, Immunostaining

    MLKL in myofibroblasts affects the activity of macrophages. ( A ) The secretion of IL-6, NO and TNF-α in MLKL +/+ M1ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( B ) The secretion of IL-10, arginase and Ym1 in MLKL +/+ M2ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( C )The skin wound tissues of MLKL +/+ and MLKL −/− mice were subjected to immunofluorescence staining for arginase (red), Ym1 (green) and nuclear (DAPI, blue) on the 5, 7 and 10th days after wound injury. Merge represents the composite picture of target protein and nuclear. The immunofluorescence staining was imaged by fluorescence microscopy (Zeiss LSM 800 laser, ×200 magnification), and the mean value of fluorescence intensity was used to perform calculation (a. u., arbitrary unit). ( D ) The protein expression of arginase and Ym1 in wound tissues of MLKL +/+ and MLKL −/− mice were determined by western blot. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean ± SD of multiple independent experiments and analyzed by Student t test or one-way ANOVA followed by Tukey’s multiple comparisons test ( n = 4–6). * P < 0.05, ** P < 0.01, *** P < 0.001. Scale label = 20 μm.

    Journal: Scientific Reports

    Article Title: MLKL is involved in the regulation of skin wound healing and interplay between macrophages and myofibroblasts in mice

    doi: 10.1038/s41598-025-97729-2

    Figure Lengend Snippet: MLKL in myofibroblasts affects the activity of macrophages. ( A ) The secretion of IL-6, NO and TNF-α in MLKL +/+ M1ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( B ) The secretion of IL-10, arginase and Ym1 in MLKL +/+ M2ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( C )The skin wound tissues of MLKL +/+ and MLKL −/− mice were subjected to immunofluorescence staining for arginase (red), Ym1 (green) and nuclear (DAPI, blue) on the 5, 7 and 10th days after wound injury. Merge represents the composite picture of target protein and nuclear. The immunofluorescence staining was imaged by fluorescence microscopy (Zeiss LSM 800 laser, ×200 magnification), and the mean value of fluorescence intensity was used to perform calculation (a. u., arbitrary unit). ( D ) The protein expression of arginase and Ym1 in wound tissues of MLKL +/+ and MLKL −/− mice were determined by western blot. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean ± SD of multiple independent experiments and analyzed by Student t test or one-way ANOVA followed by Tukey’s multiple comparisons test ( n = 4–6). * P < 0.05, ** P < 0.01, *** P < 0.001. Scale label = 20 μm.

    Article Snippet: Ym1 , Rabbit polyclonal , 45 kDa , 1:1000 , Stemcell technologies , 60,130.

    Techniques: Activity Assay, Immunofluorescence, Staining, Fluorescence, Microscopy, Expressing, Western Blot, Control, Software

    MLKL in myofibroblasts affects macrophages activities through PGE 2 . ( A ) The production of IL-6, NO and TNF-α in MLKL +/+ M1 macrophages after MLKL −/− MFbCM treatment or MLKL −/− MFbCM plus PGE 2 treatment. ( B ) The production of IL-10, arginase and Ym1 in MLKL +/+ M2ø after MLKL −/− MFbCM treatment or MLKL −/− MFbCM plus PGE 2 treatment. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean ± SD and were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test ( n = 4–6). * P < 0.05, ** P < 0.01, *** P < 0.001. ns not significant.

    Journal: Scientific Reports

    Article Title: MLKL is involved in the regulation of skin wound healing and interplay between macrophages and myofibroblasts in mice

    doi: 10.1038/s41598-025-97729-2

    Figure Lengend Snippet: MLKL in myofibroblasts affects macrophages activities through PGE 2 . ( A ) The production of IL-6, NO and TNF-α in MLKL +/+ M1 macrophages after MLKL −/− MFbCM treatment or MLKL −/− MFbCM plus PGE 2 treatment. ( B ) The production of IL-10, arginase and Ym1 in MLKL +/+ M2ø after MLKL −/− MFbCM treatment or MLKL −/− MFbCM plus PGE 2 treatment. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean ± SD and were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test ( n = 4–6). * P < 0.05, ** P < 0.01, *** P < 0.001. ns not significant.

    Article Snippet: Ym1 , Rabbit polyclonal , 45 kDa , 1:1000 , Stemcell technologies , 60,130.

    Techniques: Control, Software

    Antibodies used in Immunofluorescence staining.

    Journal: Scientific Reports

    Article Title: MLKL is involved in the regulation of skin wound healing and interplay between macrophages and myofibroblasts in mice

    doi: 10.1038/s41598-025-97729-2

    Figure Lengend Snippet: Antibodies used in Immunofluorescence staining.

    Article Snippet: Ym1 , Rabbit polyclonal , 45 kDa , 1:1000 , Stemcell technologies , 60,130.

    Techniques: Immunofluorescence, Staining, Concentration Assay

    Antibodies used in Western blot.

    Journal: Scientific Reports

    Article Title: MLKL is involved in the regulation of skin wound healing and interplay between macrophages and myofibroblasts in mice

    doi: 10.1038/s41598-025-97729-2

    Figure Lengend Snippet: Antibodies used in Western blot.

    Article Snippet: Ym1 , Rabbit polyclonal , 45 kDa , 1:1000 , Stemcell technologies , 60,130.

    Techniques: Western Blot, Concentration Assay

    Antibodies used in immunofluorescence staining

    Journal: Cell Communication and Signaling : CCS

    Article Title: NLRP3: a key regulator of skin wound healing and macrophage-fibroblast interactions in mice

    doi: 10.1186/s12964-025-02063-9

    Figure Lengend Snippet: Antibodies used in immunofluorescence staining

    Article Snippet: Ym1 , Rabbit polyclonal , , 1:50 , Stemcell technologies , 60,130.

    Techniques: Immunofluorescence

    Antibodies used in Western blot

    Journal: Cell Communication and Signaling : CCS

    Article Title: NLRP3: a key regulator of skin wound healing and macrophage-fibroblast interactions in mice

    doi: 10.1186/s12964-025-02063-9

    Figure Lengend Snippet: Antibodies used in Western blot

    Article Snippet: Ym1 , Rabbit polyclonal , , 1:50 , Stemcell technologies , 60,130.

    Techniques: Western Blot, Concentration Assay

    Comparison of M1/M2 macrophages in liver tissues. ( A ) Immunohistochemistry staining for Ym1 and CD68. ( B ) The number of positive cells for Ym1 and CD68 was quantified. The Ym1-to-CD68 ratio was analyzed. Values are presented as means ± standard deviations. n = 13–20 per experiment. * p < 0.05.

    Journal: Diseases

    Article Title: Dasatinib and Quercetin as Senolytic Drugs Improve Fat Deposition and Exhibit Antifibrotic Effects in the Medaka Metabolic Dysfunction-Associated Steatotic Liver Disease Model

    doi: 10.3390/diseases12120317

    Figure Lengend Snippet: Comparison of M1/M2 macrophages in liver tissues. ( A ) Immunohistochemistry staining for Ym1 and CD68. ( B ) The number of positive cells for Ym1 and CD68 was quantified. The Ym1-to-CD68 ratio was analyzed. Values are presented as means ± standard deviations. n = 13–20 per experiment. * p < 0.05.

    Article Snippet: IHC was performed using rabbit anti-p21 polyclonal antibody (orb3599, Biorbyt, Cambridge, UK), rabbit anti-γH2AX polyclonal antibody (ab11174, Abcam, Cambridge, UK), rabbit anti-LMNB1 polyclonal antibody (ab16048, Abcam, Cambridge, UK), rabbit anti-Ym1 polyclonal antibody (#60130, STEMCELL technology, Vancouver, BC, Canada), rabbit anti-CD68 polyclonal antibody (ab125212, Abcam, Cambridge, UK), Vectastain Elite ABC Rabbit IgG kit (PK-6101; Vector Laboratories, Burlingame, CA, USA), and DAB chromogen tablet (Muto Pure Chemicals, Tokyo, Japan) for IHC.

    Techniques: Comparison, Immunohistochemistry, Staining